کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7617461 1494070 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fast analysis of polyphenols in royal jelly products using automated TurboFlow™-liquid chromatography-Orbitrap high resolution mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fast analysis of polyphenols in royal jelly products using automated TurboFlow™-liquid chromatography-Orbitrap high resolution mass spectrometry
چکیده انگلیسی
This study describes the development of a novel, simple and fast analytical method for the detection and quantification of polyphenols in royal jelly products, using an in-house database containing more than 50 compounds. The extraction method consisted of sample dilution, followed by a fast on-line system composed of turbulent flow chromatography (TurboFlow™) coupled to liquid chromatography (LC)-Exactive-Orbitrap analyzer. The total run time was 18 min, including automated extraction, analytical chromatography and re-equilibration. The method was validated obtaining limits of quantification (LOQ) ranging from 10 to 150 μg/kg. The linearity range was up to 2000 μg/L and determination coefficients (R2) were higher than 0.994. Adequate recoveries were obtained at three concentration levels (500, 1000 and 2000 μg/kg). This method was applied to the analysis of nine samples and the concentration of polyphenols ranged from 14 (apigenin) to 18,936 μg/kg (ferulic acid).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 973, 15 December 2014, Pages 17-28
نویسندگان
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