کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617461 | 1494070 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fast analysis of polyphenols in royal jelly products using automated TurboFlowâ¢-liquid chromatography-Orbitrap high resolution mass spectrometry
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
This study describes the development of a novel, simple and fast analytical method for the detection and quantification of polyphenols in royal jelly products, using an in-house database containing more than 50 compounds. The extraction method consisted of sample dilution, followed by a fast on-line system composed of turbulent flow chromatography (TurboFlowâ¢) coupled to liquid chromatography (LC)-Exactive-Orbitrap analyzer. The total run time was 18 min, including automated extraction, analytical chromatography and re-equilibration. The method was validated obtaining limits of quantification (LOQ) ranging from 10 to 150 μg/kg. The linearity range was up to 2000 μg/L and determination coefficients (R2) were higher than 0.994. Adequate recoveries were obtained at three concentration levels (500, 1000 and 2000 μg/kg). This method was applied to the analysis of nine samples and the concentration of polyphenols ranged from 14 (apigenin) to 18,936 μg/kg (ferulic acid).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 973, 15 December 2014, Pages 17-28
Journal: Journal of Chromatography B - Volume 973, 15 December 2014, Pages 17-28
نویسندگان
Noelia López-Gutiérrez, MarÃa del Mar Aguilera-Luiz, Roberto Romero-González, José Luis MartÃnez Vidal, Antonia Garrido Frenich,