کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7617836 1494084 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of LC-MS determination method and back-propagation ANN pharmacokinetic model of corynoxeine in rat
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of LC-MS determination method and back-propagation ANN pharmacokinetic model of corynoxeine in rat
چکیده انگلیسی
Corynoxeine(CX), isolated from the extract of Uncaria rhynchophylla, is a useful and prospective compound in the prevention and treatment for vascular diseases. A simple and selective liquid chromatography mass spectrometry (LC-MS) method was developed to determine the concentration of CX in rat plasma. The chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm × 150 mm, 5 μm) column with acetonitrile-0.1% formic acid in water as mobile phase. Selective ion monitoring (SIM) mode was used for quantification using target ions m/z 383 for CX and m/z 237 for the carbamazepine (IS). After the LC-MS method was validated, it was applied to a back-propagation artificial neural network (BP-ANN) pharmacokinetic model study of CX in rats. The results showed that after intravenous administration of CX, it was mainly distributed in blood and eliminated quickly, t1/2 was less than 1 h. The predicted concentrations generated by BP-ANN model had a high correlation coefficient (R > 0.99) with experimental values. The developed BP-ANN pharmacokinetic model can be used to predict the concentration of CX in rats
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 959, 15 May 2014, Pages 10-15
نویسندگان
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