کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617928 | 1494089 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Targeting deeper the human serum fucome by a liquid-phase multicolumn platform in combination with combinatorial peptide ligand libraries
ترجمه فارسی عنوان
هدف قرار دادن عمیق تر فوکوم سرم انسان توسط یک پلات مایع فاز چند قطبی در ترکیب با کتابخانه های لیگاند پپتید ترکیبی
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کلمات کلیدی
LACDEPsAIBNGMM2,2′-azobis(isobutyronitrile)LTAAALTFACPLLGlcNAcPETAFucoseFucLotus tetragonolobus agglutininRPc - RPCAleuria aurantia lectin - آلووریا آرنتیا لکتینTrifluoroacetic acid - اسید Trifluoroaceticimmunoglobulins - ایمونوگلوبولین هاMonolithic columns - ستونهای یکپارچهFucosylation - فوکسیلینگN-acetylglucosamine - نیتستیگلوکوزامینDifferentially expressed proteins - پروتئین های متفاوتی بیان شده استCombinatorial peptide ligand library - کتابخانه لیگاند پپتید ترکیبیReversed phase chromatography - کروماتوگرافی فازی معکوسLectin affinity chromatography - کروماتوگرافی وابسته به لکتینGal - گالGalactose - گالاکتوزمی glycoproteins - گلیکوپروتئین ها
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Combinatorial peptide ligand library (CPLL) was evaluated as an off line step to narrow the differences of protein concentration in human serum prior to the capturing of human fucome from disease-free and breast cancer sera by a multicolumn platform via lectin affinity chromatography (LAC) followed by the fractionation of the captured glycoproteins by reversed phase chromatography (RPC). Two monolithic lectin columns specific to fucose, namely Aleuria aurantia lectin (AAL) and Lotus tetragonolobus agglutinin (LTA) columns were utilized to capture the fucome, which was subsequently fractionated by RPC yielding desalted fractions in volatile acetonitrile-rich mobile phase, which after vacuum evaporation were subjected to tryptic digestion prior to LC-MS/MS analysis. AAL has a strong affinity towards core fucosylated N-glycans and has a weak binding towards fucose in the outer arm while LTA can bind to glycans having fucose present in the outer arm. The combined strategy consisting of the CPLL, multicolumn platform and LC-MS/MS analysis permitted the identification of the differentially expressed proteins (DEPs) in breast cancer serum yielding 58 DEPs in both the LTA and AAL fractions with 6 DEPs common to both lectins. 17 DEPs were of the low abundance type, 16 DEPs of the borderline abundance type, 4 DEPs of the medium abundance type and 15 DEPs of the high abundance type. The remaining 6 DEPs are of unknown concentration. Only proteins exhibiting 99.9% protein identification probability, 95% peptide identification probability, and a minimum of 5 unique peptides were considered in finding the DEPs via scatterplots.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 951â952, 1 March 2014, Pages 135-142
Journal: Journal of Chromatography B - Volumes 951â952, 1 March 2014, Pages 135-142
نویسندگان
Subhashini Selvaraju, Ziad El Rassi,