کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7618038 | 1494095 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of a quantitative liquid chromatography tandem mass spectrometry assay for pristimerin in rat plasma
ترجمه فارسی عنوان
توسعه و اعتبار سنجی طیف سنجی جرم کروماتوگرافی مایع کم عمق برای پرستیمیرین در پلاسمای موش
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A sensitive, rapid and simple LC-MS/MS analysis method was developed and validated for the determination of pristimerin (PR) in rat plasma. Protein precipitation with four volumes of acetonitrile as the precipitation reagent was used as the sample preparation method. The analysis process was performed on a Merck ZIC-HILIC column with the mobile phase of acetonitrile-water (containing 5Â mM ammonium formate, pH 2.8) (85:15, v/v). PR (m/z 465.3-201.1) and glycyrrhetinic acid (internal standard, m/z 471.5-177.1) were monitored under positive electrospray ionization in multiple reaction monitoring (MRM) mode. Retention time of PR and IS was 2.45Â min and 2.4Â min, respectively. The limit of detection was 0.5Â ng/mL and the linear range was 1-500Â ng/mL. The intra-day and inter-day precision were 2.89-6.27% and 4.91-8.98%, and the intra-day and inter-day accuracy ranged from â5.81% to 8.64% and â7.37% to 9.57%, respectively. The matrix effects and absolute recovery ranged from 89.3% to 92.4% and 88.7% to 92.8%, respectively. The method has been successfully applied to the determination of PR concentration in rat plasma after intravenous administration (0.5Â mg/kg).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 941, 15 December 2013, Pages 25-30
Journal: Journal of Chromatography B - Volume 941, 15 December 2013, Pages 25-30
نویسندگان
Xin Luan, Ying-Yun Guan, Ya-Rong Liu, Chao Wang, Mei Zhao, Qin Lu, Ya-Bin Tang, Xiao-Lin Wang, Chao Fang, Hong-Zhuan Chen,