Development and validation of a LC-MS/MS method for the quantification of the regioisomers of dihydroxybutylether in human plasma

► This is the first LC-MS/MS method to determine DHBE regioisomers in human plasma. ► Five DHBE isomers were separated and determined by this method within 16.5 min. ► This method is simple and sensitive and easily reproducible. ► It was applied to a pharmacokinetic study of DHBE regioisomers in humans.