Simple HPLC method for cefazolin determination in human serum - validation and stability testing

► Simple and low-cost method for cefazolin in serum suitable for labs with basic HPLC. ► Satisfactorily validated fulfilling new EMA guidelines (precision, accuracy, range). ► Stability (freeze-thaw, short- and long-term) tests proved repeatability and performance. ► The procedure was found resistant to potential human errors during clinical usage.