Interference by metabolites and the corresponding troubleshooting during the LC-MS/MS bioanalysis of G004, a bromine-containing hypoglycemic agent

► G004, a novel bromine-containing hypoglycemic agent with antithrombosis effect. ► The detection of an interfering metabolite in the LC-MS/MS bioanalysis of G004. ► MS conditions were optimized to eliminate the interference from the metabolite. ► Metabolite-related matrix effect was evaluated by the standard addition method. ► Pharmacokinetics of G004 in beagle dogs was reported for the first time.