Click chemistry: A route to designing and preparing pseudo-biospecific immunoadsorbent for IgG adsorption

► Exploring a novel route to a pseudo-biospecific immunoadsorbent - click chemistry. ► Preparing “clickable” reaction modules using l-histidine and original sepharose. ► Click reaction can greatly increase the immobilization efficiency of ligands. ► The 1,2,3-triazole in spacer-arm helps IgG binding without non-specific adsorption. ► The prepared immunoadsorbent exhibits excellent adsorption performance for IgG.