Liquid chromatography coupled to ion trap-tandem mass spectrometry to evaluate juvenile hormone III levels in bee hemolymph from Nosema spp. infected colonies

► JH III was determined in bee hemolymph by LC-MS/MS for the first time. ► The proposed sample treatment was fast and simple. ► A mixture of methanol and phenylthiourea was chosen to dilute bee hemolymph. ► Matrix (bee hemolymph) did not have any influence onto the analyte signal. ► The highest concentration of JH III was found in bees infected with Nosema ceranae.