Determination of celecoxib in human plasma by liquid chromatography-tandem mass spectrometry

► The sample preparation step was simplified using protein precipitation. ► The run time was shortened with a more selective LC-MS/MS procedure. ► Isotopically labeled internal standard was used to improve precision and accuracy. ► A rapid method for determination of celecoxib in human plasma has been developed. ► The method was successfully applied to a pharmacokinetic study.