کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7619036 1494140 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein characterization by LC-MS/MS may be required for the DNA identification of a fusion hemoglobin: The example of Hb P-Nilotic
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Protein characterization by LC-MS/MS may be required for the DNA identification of a fusion hemoglobin: The example of Hb P-Nilotic
چکیده انگلیسی
DNA analysis is currently the easiest way to identify a hemoglobin variant in most cases. Nevertheless, in case of complex gene rearrangements, mass spectrometry studies may be required to orientate the DNA diagnosis. The present report shows the use of mass spectrometry techniques prior to DNA analysis for the identification of the rare P-Nilotic fusion hemoglobin. Complete protein analysis is performed by liquid chromatography-tandem mass spectrometry on the abnormal globin chain isolated by reversed-phase liquid chromatography.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 883–884, 1 February 2012, Pages 172-176
نویسندگان
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