Analysis of goldenseal, Hydrastis canadensis L., and related alkaloids in urine using HPLC with UV detection

► We developed a HPLC-UV method to detect goldenseal root alkaloids. ► Testing for goldenseal prior to urine drug testing eliminates false positives. ► We extracted and detected berberine and hydrastine alkaloids from goldenseal. ► The method can be used to analyze goldenseal alkaloids in powder, liquid and urine.