Accuracy profile validation of a new method for carbon monoxide measurement in the human blood using headspace-gas chromatography-mass spectrometry (HS-GC-MS)

► We have developed a new procedure to measure CO in human blood. ► CO has never been quantified with internal labeled standard 13CO. ► We have validated the technique according SFSTP requirements and tested it on real postmortem blood samples. ► Our method is satisfactory to measure CO in human blood.