کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625758 | 1494557 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The effects of an antioxidative pentapeptide derived from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The effects of an antioxidative pentapeptide from chickpea protein hydrolysates on oxidative stress in Caco-2 and HT-29 cell lines were investigated. The bioactive pentapeptide had an amino acid sequence of Asn-Arg-Try-His-Glu (NRYHE). Caco-2 and HT-29 cells were pretreated with peptide (0.05, 0.1, 0.25 and 0.5 mg/mL) for 2 h and stimulated with 1 mM H2O2 for 6 h. The activity of three important antioxidative enzymes, catalase, glutathione reductase and glutathione peroxidase, increased in a concentration-dependent manner. It was observed that peptide treatment elevated the expression of Nrf2 mRNA and several relative genes NQO1, HO-1, γ-GCS regulated by Nrf2 compared to the positive control. The trends in HT-29 and Caco-2 cells were similar.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 7, March 2014, Pages 719-726
Journal: Journal of Functional Foods - Volume 7, March 2014, Pages 719-726
نویسندگان
Yujie Guo, Tao Zhang, Bo Jiang, Ming Miao, Wanmeng Mu,