Magnetic capture of polydopamine-encapsulated Hela cells for the analysis of cell surface proteins

Hela cells were encapsulated with polydopamine (PDA) and further coated with magnetic nanoparticles. Then, the magnetic cells and their broken fragment of the PMs could be isolated with a magnet field. Plasma membrane proteins and their complexes could be well maintained. After tryptic digestion, peptides could be isolated from the PDA shell.102