Optimised 'on demand' protein arraying from DNA by cell free expression with the 'DNA to Protein Array' (DAPA) technology

► DAPA technology enables fast, cost effective production of protein arrays. ► Optimisation of array support coatings resulted in significant performance improvement. ► Technology optimisation yields up to three times more expressed protein. ► Antibodies screened on DAPA arrays yielded results similar to those with conventionally spotted protein arrays.