The role of Yca1 in proteostasis. Yca1 regulates the composition of the insoluble proteome

► Wildtype and Yca1 knockout cells were grown under normal and heat stress conditions. ► Insoluble protein fraction was analyzed by 2D LC-MS. ► Composition of the insoluble fraction is non-specific and largely variable. ► Cdc48 level in the insoluble fraction is Yca1 dependent. ► Yca1 influences ribosomal protein function during stress.