Tubulin isoforms identified in the brain by MALDI in-source decay

► ISD fragmentation of HSA takes place preferentially via the radical pathway. ► Selectively enhancement of ISD fragmentation of tubulin via the thermal pathway. ► Identification of tubulin isotypes in tissues without any chemical modification. ► Detection of series ions from fragmentation of N and C terminus of myelin and tubulin isotypes proteins. ► Identification of α1B mouse tubulin isotype by pseudo MS/MS type experiment (T3-sequencing).