Clinical use of phosphorylated proteins in blood serum analysed by immobilised metal ion affinity chromatography and mass spectrometry

► We used IMAC and nanoLC-ESI-QTOF to analyse serum samples. ► Non-pooled serum samples from 80 volunteers were investigated individually. ► Peptides had to be detected in 2/3 technical replicates and in > 70% of all samples. ► Just under 6000 phosphopeptides corresponding to > 500 proteins detected. ► Specific examples of clinical use of phosphoproteins given.