کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7639322 | 1494821 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Accumulation of copper in the kidney of pigs fed high dietary zinc is due to metallothionein expression with minor effects on genes involved in copper metabolism
ترجمه فارسی عنوان
انباشت مس در کلیه خوک های تغذیه شده با رژیم غذایی بالا به علت بیان فلوتونیئین با اثرات جزئی بر روی ژن های متابولیسم مس
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کلمات کلیدی
ZnTAtox1CCSCTR1ATP7AADGSOD1DMT1CeruloplasminZIPsdhAsuccinate dehydrogenase subunit AZinc oxide - اکسید رویPiglets - بچه خوکGene expression - بیان ژنdivalent metal ion transporter 1 - حمل کننده 1 یون فلز متخلخلZinc transporter - حمل کننده رویCopper transporter 1 - حمل کننده مس 1Copper chaperone for superoxide dismutase - شاپرن مس برای سوپر اکسید دیسموتازdry matter - ماده خشکMetallothionein - متالوتونیئینaverage daily gain - متوسط افزایش روزانهCopper - مسbody weight - وزن بدنKidney - کلیه
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A study was conducted to determine the effect of high dietary zinc (Zn) oxide on trace element accumulation in various organs with special emphasis on the kidney. A total of 40 weaned piglets were allocated into two groups with 16 and 24 piglets each receiving a diet containing normal (NZn; 100Â mg Zn/kg) or high (HZn; 2,100Â mg Zn/kg) Zn concentration, respectively. After two weeks, eight piglets from each treatment were killed and organ samples were taken. Eight piglets from the remaining 16 pigs fed HZn diets were changed to NZn diets (CZn). All remaining piglets were killed after another two weeks for organ sampling. Trace element concentration was determined in the jejunum, liver, kidney, pancreas, bone (metacarpal IV), spleen, lung, thymus, tonsils and lymph nodes of jejunum, ileum and colon. Kidney mRNA expression of Zn transporter ZnT1 and ZIP4, genes involved in Cu metabolism (Ctr1, Atox1, SOD1, ATP7A, CCS, CP) and divalent metal ion transport (DMT1) and binding (MT-1a, MT-2b, MT-3) were determined. The Zn concentration in jejunum, liver, pancreas tissue and metacarpal IV was higher (PÂ <Â 0.05) in HZn group compared with NZn and CZn groups. Trace element concentration in organs of CZn pigs was similar to those fed NZn diets. Zn concentration in muscle, lung and lymphatic organs as thymus, tonsils, spleen and lymph nodes of jejunum, ileum and colon did not differ between the groups. Zn and Cu were positively correlated (RÂ =Â 0.67; PÂ <Â 0.05) in the kidney. No significant differences for Cu chaperones, Cu transporters and Cu-dependent factors were determined despite decreased expression of Atox1 after two weeks and increased Ctr1 expression over time in the HZn group. Expression of MT-1a, MT-2b and MT-3 were significantly higher in HZn fed pigs with most pronounced effects for MT-1a > MT-2b > MT-3. Gene expression of MTs in pigs fed CZn diets did not differ from pigs fed NZn diets. The data suggest that high dietary Zn feeding in pigs leads to Cu co-accumulation in the kidney of pigs with minor effect on genes relevant for Cu metabolism. In addition, the organ Zn and Cu accumulation is reversible after two weeks of withdrawal of high dietary Zn.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Trace Elements in Medicine and Biology - Volume 35, May 2016, Pages 1-6
Journal: Journal of Trace Elements in Medicine and Biology - Volume 35, May 2016, Pages 1-6
نویسندگان
A. Zetzsche, N. Schunter, J. Zentek, R. Pieper,