کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7692180 1496266 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Photo-triggered recognition between host and guest compounds in a giant vesicle encapsulating photo-pierceable vesicles
ترجمه فارسی عنوان
تشخیص عکس در میان ترکیب میزبان و مهمان در یک حنجره غول پیکر که حاوی حوضچه های قابل تحسین عکس است
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی
Here, we used centrifugal precipitation to construct a giant vesicle (GV) encapsulating smaller giant vesicles (GV-in-GV) which comprises a photo-resistant outer GV and a photo-pierceable inner GV; the outer GV contained a fluorescent probe (SYBR Green I) in its inner water pool, and the inner GV contained double-stranded DNA (dsDNA) in its inner water pool. The phospholipid membrane of the inner GV was made photo-pierceable by inclusion of ca. 15 mol% of a caged phospholipid in its membrane. Immediately after exposure of the GV-in-GVs to UV irradiation, strong fluorescence was detected in the inner water pool of the outer GV, indicating that dsDNA had been released from the inner GV and had complexed with the fluorescent probe. These dynamics can be recognized as a macroscopic representation of the molecular level function of a caged compound.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemistry and Physics of Lipids - Volume 210, January 2018, Pages 70-75
نویسندگان
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