Phase structure of liposome in lipid mixtures

► The relationship between the lipid mixture phase structure and the stability of gas microbubble ultrasound imaging systems was investigated. ► The phase behavior of a ternary lipid mixture in water with cosolvents was used. ► The polar PEG side chains from DPPE-PEG had a significant impact on the phase microstructure. ► The microbubbles of an ultrasound imaging system are stabilized by lipid bilayers, not micelles.