کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7694218 1496478 2016 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Deciphering single cell metabolism by coherent Raman scattering microscopy
ترجمه فارسی عنوان
تجزیه و تحلیل متابولیسم تک سلولی با میکروسکوپ پراکندگی رامان همگرا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی
Metabolism is highly dynamic and intrinsically heterogeneous at the cellular level. Although fluorescence microscopy has been commonly used for single cell analysis, bulky fluorescent probes often perturb the biological activities of small biomolecules such as metabolites. Such challenge can be overcome by a vibrational imaging technique known as coherent Raman scattering microscopy, which is capable of chemically selective, highly sensitive, and high-speed imaging of biomolecules with submicron resolution. Such capability has enabled quantitative assessments of metabolic activities of biomolecules (e.g. lipids, proteins, nucleic acids) in single live cells in vitro and in vivo. These investigations provide new insights into the role of cell metabolism in maintenance of homeostasis and pathogenesis of diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Opinion in Chemical Biology - Volume 33, August 2016, Pages 46-57
نویسندگان
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