Dark dyes-bright complexes: fluorogenic protein labeling

- Protein-dye complexes that activate fluorescence allow no-wash protein labeling.
- Quencher removal, environmental change and direct binding can activate fluorescence.
- Modifications of the chromophore and linker can influence fluorogen performance.
- Pulse-chase and order-of-addition experiments can reveal dynamic cellular changes.
- High-quality fluorogen imaging in organisms will reveal new biological processes.