Watching protein structure at work in living cells using NMR spectroscopy

► Advances in in-cell NMR spectroscopy of biomacromolecules. ► Escherichia coli, yeasts, Xenopus laevis oocytes and mammalian cell lines can be used. ► Structures, dynamics and interactions of proteins in living cells are studied. ► Proteins in the intracellular space behave differently from those in vitro.