کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7715045 | 1497443 | 2015 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a synthetic pathway to convert glucose to hydrogen using cell free extracts
ترجمه فارسی عنوان
توسعه یک راه مصنوعی برای تبدیل گلوکز به هیدروژن با عصاره های آزاد سلولی
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
چکیده انگلیسی
Sustainable production of biochemicals from cellulosic biomass is an attractive alternative to chemical production from fossil fuels. We describe the further development of a three protein synthetic pathway to convert NADPH and H+ to hydrogen using a ferredoxin-NADP+ reductase, a ferredoxin, and the [FeFe] hydrogenase from Clostridium pasteurianum at a rate greater than 14Â mmol H2Â Lâ1 hrâ1 using natural enzymes. We also demonstrate the feasibility of coupling this pathway to a cell-free extract to convert glucose to hydrogen in a potentially cost effective manner. Both of these accomplishments serve as the basis for further engineering to optimize both the yield and productivity of a low-cost cell-free process for the production of highly reduced biochemicals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 40, Issue 30, 10 August 2015, Pages 9113-9124
Journal: International Journal of Hydrogen Energy - Volume 40, Issue 30, 10 August 2015, Pages 9113-9124
نویسندگان
Franklin Lu, Phillip R. Smith, Kunal Mehta, James R. Swartz,