کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7718172 | 1497483 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of thermophilic fermentative hydrogen production by the newly isolated Caloranaerobacter azorensis H53214 from deep-sea hydrothermal vent environment
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
الکتروشیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Optimization of thermophilic fermentative hydrogen production by the newly isolated Caloranaerobacter azorensis H53214 from deep-sea hydrothermal vent environment Optimization of thermophilic fermentative hydrogen production by the newly isolated Caloranaerobacter azorensis H53214 from deep-sea hydrothermal vent environment](/preview/png/7718172.png)
چکیده انگلیسی
A unique thermophilic fermentative hydrogen-producing strain H53214 was isolated from a deep-sea hydrothermal vent environment, and identified as Caloranaerobacter azorensis based on bacterial 16S rRNA gene analysis. The optimum culture condition for hydrogen production by the bacterium, designated C. azorensis H53214, was investigated by the response surface methodology (RSM). Eight variables including the concentration of NaCl, glucose, yeast, tryptone, FeSO4 and MgSO4, initial pH and incubation temperature were screened based on the Plackett-Burman design. The results showed that initial pH, tryptone and yeast were significant variables, which were further optimized using the steepest ascent method and Box-Behnken design. The optimal culture conditions for hydrogen production were an initial pH of 7.7, 8.3 g Lâ1 tryptone and 7.9 g Lâ1 yeast. Under these conditions, the maximum cumulative hydrogen volume, hydrogen yield and maximum H2 production rate were 1.58 L H2 Lâ1 medium, 1.46 mol H2 molâ1 glucose and 25.7 mmol H2 gâ1 cell dry weight (CDW) hâ1, respectively. By comparison analysis, strain H53214 was superior to the most thermophilic hydrogen producers because of the high hydrogen production rate. In addition, the isolation of C. azorensis H53214 indicated the deep-sea hydrothermal environment might be a potential source for fermentative hydrogen-producing thermophiles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Hydrogen Energy - Volume 39, Issue 26, 3 September 2014, Pages 14154-14160
Journal: International Journal of Hydrogen Energy - Volume 39, Issue 26, 3 September 2014, Pages 14154-14160
نویسندگان
Lijing Jiang, Chuannan Long, Xiaobing Wu, Hongxiu Xu, Zongze Shao, Minnan Long,