Photofermentative hydrogen production using dark fermentation effluent of sugar beet thick juice in outdoor conditions

► DFE of thick juice is a suitable feedstock for photofermentative H2 production. ►Rb capsulatus YO3 (hup−) can produce H2 using DFE of thick juice. ► Temperature and light intensity variations affect H2 production in outdoor. ► Fe, Mo and pH adjustment of the DFE of thick juice is necessary for H2 production.