Determining the glycation site specificity of human holo-transferrin

The glycation site specificity of human diferric holo-transferrin (Tf) was evaluated using tandem mass spectrometry. The glycation pattern was different from apo-Tf, with lysine residues 103, 312 and 380 proving to be the most reactive sites in holo-Tf. An in silico approach evaluated the role of the lysine microenvironment.197