The influence of the Cys46/Cys55 disulfide bond on the redox and spectroscopic properties of human neuroglobin

UV-vis spectroelectrochemistry, cyclic voltammetry, electronic absorption spectroscopy and magnetic circular dichroism (MCD) were used to investigate the influence of the Cys46/Cys55 disulfide bond on the redox properties and on the pH-dependent conformational equilibria of human neuroglobin, a six-coordinate globin exerting a relevant neuroprotective role.224