The catalytic cycle of nitrous oxide reductase - The enzyme that catalyzes the last step of denitrification

Nitrous oxide reductase is a multicopper enzyme containing a CuA and a tetranuclear copper sulfide-bridged “CuZ center”, that catalyzes N2O reduction. “CuZ center” exists in two forms, CuZ(4Cu2S) and CuZ*(4Cu1S), with the later proven to be the relevant species in the reaction mechanism that involves CuZ0 as an intermediate species.194