کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7782683 | 1500566 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Radical scavenging activity of sulfated Bupleurum chinense polysaccharides and their effects against oxidative stress-induced senescence
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Radical scavenging activity of sulfated Bupleurum chinense polysaccharides and their effects against oxidative stress-induced senescence Radical scavenging activity of sulfated Bupleurum chinense polysaccharides and their effects against oxidative stress-induced senescence](/preview/png/7782683.png)
چکیده انگلیسی
In this study, BCPS-1, a polysaccharide previously isolated and characterized from Bupleurum chinense was chemically modified to yield two sulfated derivatives, which we designated as S-BCP1-4 and S-BCP1-8. The physicochemical properties of these sulfated derivatives were then determined by high performance liquid chromatography (HPLC), Fourier transform infrared spectrometry (FT-IR), and gas chromatography (GC), and then compared with those of BCPS-1. Furthermore, the antioxidant activities of all three polysaccharides were also evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, superoxide radical assay and hydroxyl radical assay, while their effects against H2O2-induced cellular senescence were determined using senescence-associated β-galactosidase (SA-β-gal) staining, cell cycle assay and immunoblotting in H2O2-induced mouse lung endothelial cells (MLECs). Compared to BCPS-1, S-BCP1-4 and S-BCP1-8 exhibited remarkable antioxidant effect, and in a concentration-dependent manner. They also provided stronger protection against H2O2-induced cellular senescence in MLECs. These results indicated that the sulfate group in the modified B. chinense polysaccharides might play an important role in radical scavenging and resistance to H2O2-induced senescence. These sulfated polysaccharides could be considered as novel pharmaceutical products with potential antioxidant and anti-senescence effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 192, 15 July 2018, Pages 143-149
Journal: Carbohydrate Polymers - Volume 192, 15 July 2018, Pages 143-149
نویسندگان
Haibin Tong, Xiaoli Zheng, Jianxi Song, Jian Liu, Ting Ren, Xu Zhang, Luqi Huang, Mingjiang Wu,