کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7787840 | 1500625 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deacetylation affects the physical properties and bioactivity of acemannan, an extracted polysaccharide from Aloe vera
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کلمات کلیدی
AcemannanSemi-empirical AM1 methodMDAHGFsDDATDAddH2OPBSMWCOSECDMEM3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDMSO - DMSOMTT - MTTdouble-distilled water - آب دوتاییAloe vera - آلوئه وراSize-exclusion chromatography - اندازه گیری کروماتوگرافی حذف شدهDegree of deacetylation - درجه آلودگیDeacetylation - دفع آلودگیDimethyl sulfoxide - دیمتیل سولفواکسیدVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Phosphate buffered saline - فسفات بافر شورBioactivity - فعالیت زیستیHuman gingival fibroblasts - فیبروبلاستهای لثه انسانی انسانیMolecular weight cutoff - قطع وزن مولکولیDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده Dulbecco
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Deacetylation affects the physical properties and bioactivity of acemannan, an extracted polysaccharide from Aloe vera Deacetylation affects the physical properties and bioactivity of acemannan, an extracted polysaccharide from Aloe vera](/preview/png/7787840.png)
چکیده انگلیسی
Acemannan, an acetylated polymannose from Aloe vera, induces tissue repair. We investigated the role of acemannan's acetyl-groups on its physical and biological properties. Deacetylated acemannan (DeAcAM) was prepared and characterized. The physical properties and microscopic structure of DeAcAM were evaluated using water solubility, contact angle, X-ray diffraction, and scanning-electron microscopy. The activity of DeAcAM on cell proliferation and gene expression were assessed. Acemannan and DeAcAM structures were simulated and the acemannan tetramer diad and its completely deacetylated structure were also determined. Increased acemannan deacetylation reduced its water solubility and hydrophilicity. Complete deacetylation altered acemannan's conformation to a partial crystal structure. The bioactivity of acemannan was reduced corresponding to its deacetylation. Acemannan induced cell proliferation, and VEGF and Collagen I expression; however, 100% DeAcAM did not. The simulated structures of the acemannan diad and the completely deacetylated diad were different. We conclude acetyl-groups affect acemannan's structure and physical/biological properties.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 133, 20 November 2015, Pages 556-566
Journal: Carbohydrate Polymers - Volume 133, 20 November 2015, Pages 556-566
نویسندگان
Jaroenporn Chokboribal, Wanpen Tachaboonyakiat, Polkit Sangvanich, Vithaya Ruangpornvisuti, Suwimon Jettanacheawchankit, Pasutha Thunyakitpisal,