کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7789826 | 1500641 | 2015 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of liposomal Ganoderma lucidum polysaccharide: Formulation optimization and evaluation of its immunological activity
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کلمات کلیدی
PBSGanoderma lucidum polysaccharideGLPPhytohemagglutininPHABBDFBSLPS3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideDMSO - DMSOMTT - MTTOptimization - بهينه سازيLymphocyte proliferation - تکثیر لنفوسیتDimethyl sulfoxide - دیمتیل سولفواکسیدResponse surface methodology - روش سطح پاسخRSM - روششناسی سطح پاسخfetal bovine serum - سرم جنین گاوBox-Behnken design - طرح جعبه BehnkenPhosphate buffered saline - فسفات بافر شورlipopolysaccharide - لیپوپلی ساکارید
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study was to investigate the optimizing preparation conditions of Ganoderma lucidum polysaccharide liposome (GLPL) with response surface methodology (RSM) and the immunological enhancement activity of GLPL. The immunological enhancement activity of GLPL on splenocyte proliferation was measured. The optimum formulation of GLPL, in which the ratio of soybean phospholipid to cholesterol(w/w) of 11:1, the ratio of soybean phospholipid to tween-80 (w/w) of 10.5:1 and ultrasonic time(min) of 11, had higher entrapment efï¬ciency (EE) of 71.43 ± 0.49% with spherical shape and uniform sizes. In addition, GLPL could significantly promote splenocyte proliferation singly or synergistically with PHA and LPS. That indicated that the immunological enhancement of Ganoderma lucidum polysaccharide (GLP) was significantly enhanced after encapsulation with the liposome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 117, 6 March 2015, Pages 510-517
Journal: Carbohydrate Polymers - Volume 117, 6 March 2015, Pages 510-517
نویسندگان
Zhenguang Liu, Xia Ma, Bihua Deng, Yee Huang, Ruonan Bo, Zhenzhen Gao, Yun Yu, Yuanliang Hu, Jiaguo Liu, Yi Wu, Deyun Wang,