کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7790139 | 1500643 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Viscosity based quantification of endogenous β-glucanase activity in flour
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کلمات کلیدی
HPSECHPAEC-PADDNSβ-Glucanase - β-گلوکانازCereal β-glucan - غلات β-گلوکانactivity - فعالیتMolecular weight - وزن مولکولیWeight average molecular weight - وزن مولکولی متوسط وزنViscosity - ویسکوزیته یا گرانرویhigh performance size exclusion chromatography - کروماتوگرافی خروج با اندازه کافی بالاhigh performance anion exchange chromatography with pulsed amperometric detection - کروماتوگرافی مبدل آنیون با عملکرد بالا با تشخیص آمپومتریک پالسQuantification - کوانتومی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
High molecular weight (MW) is a key parameter for cereal β-glucans physiological benefits like decreased serum cholesterol and attenuated post prandial blood glucose. However, the activity of endogenous flour enzymes during bread production results in a decrease of β-glucan MW. The depolymerization of a standard β-glucan solution by different flour extracts (wheat, barley and rye) was followed by measuring the viscosity decrease with a Rheometer. The slope of the inverse viscosity (1/η) against degradation time was used to quantify β-glucanase activity by comparison with slopes obtained with known concentrations of the β-glucanase Lichenase. Results correlated well with depolymerization rates estimated by HPSEC. The viscosity based method is rapid (20 min per sample), accurate (â¤6% variation), and a powerful screening tool for identifying flour fractions with low β-glucanase activity, treatments that can inactivate β-glucanases in flour, or the development of β-glucanase inhibitors for the use in e.g. bread making.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 115, 22 January 2015, Pages 104-111
Journal: Carbohydrate Polymers - Volume 115, 22 January 2015, Pages 104-111
نویسندگان
Anne Rieder, Simon Ballance, Svein Halvor Knutsen,