کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7791060 1500646 2014 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CTAB turbidimetric method for assaying hyaluronic acid in complex environments and under cross-linked form
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
CTAB turbidimetric method for assaying hyaluronic acid in complex environments and under cross-linked form
چکیده انگلیسی
The cetyltrimethylammonium bromide turbidimetric method (CTM) has been developed to quantify the hyaluronic acid (HA) in complex media to overcome the lack of selectivity and specificity of the standard carbazole method. The objective of this work is to assess the potential application of CTM to determine HA concentration. Factors such as duration of incubation, linearity range, HA size and form (natural linear HA or cross linked HA), pH and ionic environment impact were investigated. The incubation time was set to 10 min and the calibration curve was linear up to 0.6 g L−1. The quantitative method was relevant whatever the HA size and form, and also for a wide range of conditions. The robustness of the CTM added to its high specificity and simplicity demonstrated that the CTM is a valuable method that would be an interesting substitute to the carbazole assay for HA quantification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 112, 4 November 2014, Pages 102-108
نویسندگان
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