کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7792564 | 1500655 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis and characterization of PEG-conjugated quaternized chitosan and its application as a gene vector
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Synthesis and characterization of PEG-conjugated quaternized chitosan and its application as a gene vector Synthesis and characterization of PEG-conjugated quaternized chitosan and its application as a gene vector](/preview/png/7792564.png)
چکیده انگلیسی
Poly(ethylene glycol)-conjugated N-(2-hydroxy) propyl-3-trimethyl ammonium chitosan chloride (PHTAC) derivatives were prepared by incorporating PEG molecules onto quaternized chitosan backbone. The copolymers were characterized by FTIR, 1H NMR and XRD. Agarose gel retardation assay indicated that PHTAC had good plasmid DNA (pDNA) binding capability and the particle sizes of PHTAC/pDNA complexes determined by DLS were about 200Â nm. Cytotoxicity assays in HeLa and 293T cells showed that PHTAC had low cytotoxicity. In vitro luciferase assay showed that PHTAC with PEGylation degree of 9% (PHTAC-1) had good transfection efficiency about 5.3-fold higher than quaternized chitosan, which was comparable with PEI (25Â kDa). These results suggest that PHTAC-1 is a promising candidate as an efficient nonviral gene vector.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 103, 15 March 2014, Pages 566-572
Journal: Carbohydrate Polymers - Volume 103, 15 March 2014, Pages 566-572
نویسندگان
Xi Zhang, Juan Yao, Lihong Zhang, Jianguo Fang, Fengling Bian,