کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7792646 1500657 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Design and formulation of trimethylated chitosan-graft-poly(ɛ-caprolactone) nanoparticles used for gene delivery
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Design and formulation of trimethylated chitosan-graft-poly(ɛ-caprolactone) nanoparticles used for gene delivery
چکیده انگلیسی
The ideal gene polyplexes should have a subtle balance between polyplex stability to protect DNA against nucleases, and polyplex instability to permit DNA dissociation inside cells. In this research, low molecular weight trimethylated chitosan was chemically modified with poly(ɛ-caprolactone). Owing to the amphiphilic character, trimethylated chitosan-graft-poly(ɛ-caprolactone) (TMC-g-PCL) formed nanoparticles in aqueous media. TMC-g-PCL nanoparticles could effectively condense pDNA into polyplexes about 200 nm in size. The TMC-g-PCL/DNA polyplexes were stable in physiological salt condition and showed high uptake efficiency probably due to the increasing cell membrane-carrier interaction as a result of hydrophobic modification. However, the high degree of quaternization influenced the buffer capacity of TMC-g-PCL and led to a reduction in the release from the lysosomes. By adding chloroquine to exclude the limitation of lysosome escape, the transfection efficiency of TMC-g-PCL/DNA polyplexes was similar to that of PEI/DNA polyplexes. This study demonstrated the potential of TMC-g-PCL/DNA nanoparticles as an efficient carrier for gene delivery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 101, 30 January 2014, Pages 104-112
نویسندگان
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