Conjugation of α-amylase with dextran for enhanced stability: Process details, kinetics and structural analysis

► Effect of parameters of conjugation of α-amylase and dextran on α-amylase stability. ► Enhanced thermal and pH stability of α-amylase on conjugation with dextran. ► Conjugate showed lower inactivation rate constant with increased half life. ► Conjugation of α-amylase with dextran showed helix to turn conversion. ► Importance of covalent bonding for conjugate formation and its stability.