Characterization of a laminaribiose phosphorylase from Acholeplasma laidlawii PG-8A and production of 1,3-β-d-glucosyl disaccharides

► We discovered a laminaribiose phosphorylase (ACL0729) from Acholeplasma laidlawii. ► Six 1,3-β-d-glucosyl disaccharides were synthesized by reverse phosphorolysis. ► d-Glucose and 2-deoxy-d-arabino-hexopyranose caused competitive substrate inhibition.