کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7851339 | 1508851 | 2016 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Carbon nanotube - Protamine hybrid: Evaluation of DNA cell penetration
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We propose here a novel nanovector based on single-walled carbon nanotubes (SWCNT) functionalized by protamine. Our results based on qPCR methods clearly indicate that protamine-SWCNT/DNA hybrids were much more successful than ammonium-SWCNT/DNA hybrids to cross cell membrane and penetrate into cells. We also studied the cellular internalization mechanism of our complexes and showed that endocytosis was strongly involved. Finally, we proposed molecular dynamic simulations to understand the formation of the protamine-SWCNT/DNA complex and to extract the interaction responsible for its deliverance upon cell membrane.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbon - Volume 96, January 2016, Pages 742-752
Journal: Carbon - Volume 96, January 2016, Pages 742-752
نویسندگان
Cécile Caoduro, Raoudha Kacem, Khaoula Boukari, Fabien Picaud, Claire-Hélène Brachais, David Monchaud, Christophe Borg, Hatem Boulahdour, Tijani Gharbi, Régis Delage-Mourroux, Eric Hervouet, Marc Pudlo,