کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7866283 | 1509130 | 2018 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polymeric chitosan-glycolipid nanocarriers for an effective delivery of marine carotenoid fucoxanthin for induction of apoptosis in human colon cancer cells (Caco-2 cells)
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Fucoxanthin (FUCO), a marine carotenoid is photo-, and thermo-labile and poorly bioavailable due to its lipophilicity. Hence, we developed a chitosan (CS)â¯+â¯glycolipid (GL) nanogels (NGs) to increase cellular uptake and anticancer efficacy of FUCO (10â¯Î¼M) in human colon cells (Caco-2). Effect of FUCO loaded in NGs with/with no GL was studied in comparison with micellar FUCO. Results showed that the cell viability was lower (pâ¯<â¯0.05) in NGsâ¯+â¯GL (50.5%) compared to NGs (-GL) (66.5%) and the mixed micelles (72.5%) groups over 48â¯h exposure. An enhanced reactive oxygen species (ROS) generation was evident in NGsâ¯+â¯GL (379.2%) group compared to NGs (âGL) and mixed micelles groups. Further, induction of apoptosis with an increased chromatin condensation and DNA fragmentation as evidenced in DAPI staining and DNA ladder assay were higher in NGsâ¯+â¯GL group than other groups. Down-regulation of Bcl-2 (6.6 folds) was higher in NGsâ¯+â¯GL group compared to NGs (âGL) (1.94 fold) and mixed micelles (1.19 fold) groups. Higher Bax up-regulation in NGsâ¯+â¯GL compared to other groups supports the Bcl-2 down regulation. Mitochondrial membrane polarisation (ÎΨm) was higher in NGsâ¯+â¯GL group (2.46 fold) compared to NGs (âGL) (1.91 fold) and mixed micelles (1.26 fold) groups. The cellular FUCO uptake illustrated a positive correlation between its level (pmol/106 cells) in NGsâ¯+â¯GL (758.3) and enhanced caspase-3 activity (25.8 folds). This could be the reason for an increased apoptotic activity in NGsâ¯+â¯GL group than other groups. Results demonstrate that delivery of FUCO in NGsâ¯+â¯GL carrier aids cellular uptake and chemotherapeutic potential of FUCO. Results further demonstrate, for the first time, higher anti-cancer activity of FUCO loaded in NGsâ¯+â¯GL and the effect was through ROS generation via a caspase dependent mechanism in Caco-2 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 91, 1 October 2018, Pages 785-795
Journal: Materials Science and Engineering: C - Volume 91, 1 October 2018, Pages 785-795
نویسندگان
Hindupur Ravi, Nawneet Kurrey, Yuki Manabe, Tatsuya Sugawara, Vallikannan Baskaran,