کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7867378 | 1509153 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Three-dimensional chitin rings from body segments of a pet diplopod species: Characterization and protein interaction studies
ترجمه فارسی عنوان
حلقه های سه بعدی کیتین از بخش های بدن یک گونه از حیوانات دیپلوپ حیوانات: مطالعات متقابل و تعامل پروتئین
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
چکیده انگلیسی
Physicochemical characterization of new chitin isolates can provide valuable insights into designing of biomimetic materials. Chitin isolates with a definite three-dimensional (3D) structure can exhibit characteristics that distinguish them from other chitin specimens that are in form of powder or flakes without a definite and uniform shape. Herein, 3D chitin rings were produced from body segments of a diplopod (Archispirostreptus gigas) inhabiting tropical regions. This organism is cultured easily and can reach 38 cm in length, which makes it a suitable source for isolation of chitin. The chitin rings were characterized via TGA, FT-IR, SEM and XRD analyses. Enzymatic digestion test with chitinase demonstrated that chitin isolates had high purity (digestion rate: 97.4%). The source organism had high chitin content; 21.02 ± 2.23% on dry weight. Interactions of the chitin rings with bovine serum albumin (BSA) protein were studied under different conditions (pH: 4.0-8.0, chitin amount: 6-14 mg, contact time: 30-360 min, protein concentration: 0.2-1 mg/mL). The highest BSA adsorption was observed at pH 5.0 at 20 °C. The adsorption equilibrium data exhibited a better fit to Langmuir adsorption and the pseudo-first order kinetic models. The findings presented here can be useful for further studies aiming to develop biocompatible and nontoxic biomaterials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 68, 1 November 2016, Pages 716-722
Journal: Materials Science and Engineering: C - Volume 68, 1 November 2016, Pages 716-722
نویسندگان
Murat Kaya, Povilas MulerÄikas, Idris Sargin, Sonata KazlauskaitÄ, Vykintas Baublys, Bahar Akyuz, Esra Bulut, Vaida TubelytÄ,