کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7870143 1509186 2014 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Local microarchitecture affects mechanical properties of deposited extracellular matrix for osteonal regeneration
ترجمه فارسی عنوان
میکرواراسکوری محلی بر خواص مکانیکی ماتریکس خارج سلولی رسوب شده برای بازسازی استون تاثیر می گذارد
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی
Multiple biomimetic approaches have been attempted to accelerate the regeneration of functional bone tissue. While most synthetic scaffolds are designed to mimic the architecture of trabecular bone, in the current study, cortical bone-like extracellular matrix was regenerated in vitro within organized structures. Biphasic calcium phosphate (BCaP) and hydroxyapatite (HAp) scaffolds were developed with longitudinal microchannels (250 μm diameter) that resembled native osteons in cortical bone. BCaP and HAp scaffolds had a compressive strength of 7.61 ± 1.42 and 9.98 ± 0.61 MPa respectively. The constructs were investigated in vitro to evaluate the organization and stiffness of the extracellular matrix (ECM) formed by human fetal osteoblasts (HFObs) cultured inside the microchannels. The ECM deposited on the BCaP scaffolds was found to have a higher micro-hardness (h) (1.93 ± 0.40 GPa) than the ECM formed within the HAp microchannels (h = 0.80 ± 0.20 GPa) (p < 0.05) or native bone (h = 0.47-0.74 GPa). ECM deposition within the microchannels resembled osteoid organization and showed a significant increase in both osteoid area and thickness after 24 days (p < 0.001). These observations indicate that controlled microarchitecture, specifically cylindrical microchannels, plays a fundamental role in stimulating the appropriate cellular response aimed at recreating organized, cortical bone-like matrix. These findings open the door for researchers to develop a new generation of cortical bone scaffolds that can restore strong, organized bone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 35, 1 February 2014, Pages 122-133
نویسندگان
, , , , ,