Multi-layered functional membranes for periodontal regeneration: Preparation and characterization

Multi-layered biodegradable membranes (MLMs) were fabricated by combining electrospinning and solvent casting/particulate leaching methods. MLMs possess three layers of different functional properties; poly (caprolactone) (PCL)/nano-hydroxyapatite core layer and PCL/collagen, PCL/collagen-bone morphogenic protein 7 (BMP 7) outer layers on each side of the core layer. MC3T3-E1 cell culture tests showed that osteoblastic differentiation was enhanced on PCL/collagen BMP 7 layer. This facile method presents great potential for fabrication of multi-functional barrier membranes for periodontal regeneration as well as scaffolds possessing different properties to mimic complex extra cellular matrix structures with stable integrity.