Raman spectroscopy study of the effect of urea, uric acid and creatinine on steric configuration of bovine hemoglobin using SERS-active BN nanosheets/Ag nanoparticles hybrids

Herein surface-enhanced Raman scattering active boron nitride nanosheets/Ag nanoparticles hybrids (BN/AgNPs) has been synthesized by a wet chemistry procedure to study the effect of urea, uric acid and creatinine on steric configuration of bovine hemoglobin. XRD analysis indicates that BN/AgNPs hybrids have been successfully prepared. TEM analysis shows that as-prepared BN/AgNPs hybrids are layer-structure and the AgNPs are randomly distributed on the layered BN. Raman study shows that urea, uric acid and creatinine can destroy the steric configuration of bovine hemoglobin to some extent. The research results could be useful for biomedical application and may open an avenue to the exploration of factors affecting the demic protein structure.