کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8209 581 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Non-viral gene delivery nanoparticles based on Poly(β-amino esters) for treatment of glioblastoma
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Non-viral gene delivery nanoparticles based on Poly(β-amino esters) for treatment of glioblastoma
چکیده انگلیسی

Glioblastoma (GB) is currently characterized by low survival rates and therapies with insufficient efficacy. Here, we describe biodegradable polymers that can deliver genes to primary GB cells as well as GB tumor stem cells in vitro with low non-specific toxicity and transfection efficiencies of up to 60.6 ± 5% in normal (10%) serum conditions. We developed polymer-DNA nanoparticles that remained more stable in normal serum and could also be stored for at least 3 months in ready-to-use form with no measurable decrease in efficacy, expanding their potential in a practical or clinical setting. A subset of polymers was identified that shows a high degree of specificity to tumor cells compared with healthy astrocytes and human neural stem cells when cultured (separately or in co-culture), yielding higher transfection in GB cells while having little to no apparent effect on healthy cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 32, Issue 23, August 2011, Pages 5402–5410
نویسندگان
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