Simultaneous quantitation of oxidized and reduced glutathione via LC-MS/MS: An insight into the redox state of hematopoietic stem cells

- LC-MS/MS method for the simultaneous analysis of oxidized and reduced glutathione.
- The method is validated based on FDA guidelines in vitro and in vivo systems.
- Detection of glutathione in rare HSPCs and drug induced variations of GSH levels.
- ABCC1 expression alters intracellular glutathione concentrations within HSPCs.
- Evaluation of glutathione as a potential biomarker for hematopoietic malignancy.