کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8268405 | 1534954 | 2015 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Pyridoxamine protects proteins from damage by hypohalous acids in vitro and in vivo
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ECMCIDTFEtrifluoroethanolLC–MS/MS - LC-MS / MSCollision-induced dissociation - اختلاف ناشی از برخوردHypobromous acid - اسید هیپوبرمhypochlorous acid - اسید هیپوکلریدهDiabetes - بیماری قندposttranslational modifications - تغییرات posttranslationalFree radicals - رادیکال آزادExtracellular matrix - ماتریکس خارج سلولیNephropathy - نفروپاتیGuanidinium hydrochloride - هیدروکلراید گوانیدینpyridoxamine - پیریدوکسامینliquid chromatography coupled with tandem mass spectrometry - کروماتوگرافی مایع همراه با طیف سنجی جرمی دو طرفه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Diabetes is characterized, in part, by activation of toxic oxidative and glycoxidative pathways that are triggered by persistent hyperglycemia and contribute to diabetic complications. Inhibition of these pathways may benefit diabetic patients by delaying the onset of complications. One such inhibitor, pyridoxamine (PM), had shown promise in clinical trials. However, the mechanism of PM action in vivo is not well understood. We have previously reported that hypohalous acids can cause disruption of the structure and function of renal collagen IV in experimental diabetes (K.L. Brown et al., Diabetes64:2242-2253, 2015). In the present study, we demonstrate that PM can protect protein functionality from hypochlorous and hypobromous acid-derived damage via a rapid direct reaction with and detoxification of these hypohalous acids. We further demonstrate that PM treatment can ameliorate specific hypohalous acid-derived structural and functional damage to the renal collagen IV network in a diabetic animal model. These findings suggest a new mechanism of PM action in diabetes, namely sequestration of hypohalous acids, which may contribute to known therapeutic effects of PM in human diabetic nephropathy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 89, December 2015, Pages 83-90
Journal: Free Radical Biology and Medicine - Volume 89, December 2015, Pages 83-90
نویسندگان
Hartman Madu, Josh Avance, Sergei Chetyrkin, Carl Darris, Kristie Lindsey Rose, Otto A. Sanchez, Billy Hudson, Paul Voziyan,