کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8268889 | 1534954 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human serum determination and in vitro anti-inflammatory activity of the vitamin E metabolite α-(13'-hydroxy)-6-hydroxychroman
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کلمات کلیدی
α-TTPCEHCtocopheryl quinoneLCMiNOSCYP4F2cox2inflammation - التهاب( توروم) TOH - توTocotrienol - توکوتیرنولTocopherol - توکوفرولcytochrome P-450 - سیتوکروم P-450Cytokines - سیتوکین هاCyclooxygenase-2 - سیکلوکوکسیژناز2inducible form of nitric oxide synthase - فرم القایی سیتازاس اکسید نیتریک استVitamin E - ویتامین E α-Tocopherol transfer protein - پروتئین انتقال آلفا توکوفرول
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cytochrome P450-derived long-chain metabolites are gaining increasing interest as bioactive intermediates of vitamin E. In this study we first report on the HPLC-ECD and GC-MS analysis in human serum of the earliest metabolite of this vitamin, namely α-(13'-hydroxy)-6-hydroxychroman (α-13'-OH). The two chromatographic procedure are sensitive enough (LOQ of 10 nM) to measure α-13'-OH after hexane extraction of 1 ml of sample obtained from healthy volunteers supplemented for 1-week with 1000 IU/d (671 mg/d) RRR-α-tocopherol. The observed concentrations ranged between 15 and 50 nM, with minor differences between fasting and 4-hr post-meal state. Baseline (non-supplemented state) levels of 7.2±1.6 nM were observed extracting higher volumes of serum. Biological effects of α-13'-OH investigated for the first time in RAW264.7 murine macrophages involved transcriptional control of inflammatory cytokines, and transcriptional and functional regulation of COX2 and iNOS enzymes in response to lipopolysaccharides. In conclusion, here we present the first quantitative evaluation of serum α-13'-OH also providing early evidence of the anti-inflammatory potential of this metabolite that is worth of further investigation in the area of functional and nutraceutical implications of vitamin E metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 89, December 2015, Pages 952-962
Journal: Free Radical Biology and Medicine - Volume 89, December 2015, Pages 952-962
نویسندگان
Silvia Ciffolilli, Maria Wallert, Desirée Bartolini, Verena Krauth, Oliver Werz, Marta Piroddi, Bartolomeo Sebastiani, Pierangelo Torquato, Stefan Lorkowski, Marc Birringer, Francesco Galli,